Radioimmunoassay of 11-deoxycortisol. Specificity of antisera raised against 11-deoxycortisol-(C-4)-bovine serum albumin conjugates.
نویسندگان
چکیده
منابع مشابه
A direct radioimmunoassay for serum 11-deoxycortisol.
A simple direct radioimmunoassay for 11-deoxycortisol in serum is described. A new type of bridge heterologous 125I labeled 11-deoxycortisol was prepared and was applied to the direct radioimmunoassay. Low and high levels of 11-deoxycortisol in serum were sensitively determined by the direct radioimmunoassay.
متن کاملImproved radioimmunoassay for 11-deoxycortisol (compound S) in plasma.
We describe a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9, and 69.9 micrograms/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 micrograms/L gave inter-...
متن کاملParallel radioimmunoassay for plasma cortisol and 11-deoxycortisol.
We describe a direct, rapid, and specific procedure for the parallel radioimmunoassay for cortisol and 11-deoxycortisol in plasma. The plasma sample is used directly, after heat inactivation of the natural cortisol-binding protein. The radioimmunoassay utilizes antibodies generated in rabbits by steroids congugated at their 3-oxo position to thyroglobulin. Ammonium sulfate is used to separate b...
متن کاملPolarization fluoroimmunoassay of 11-deoxycortisol in serum and saliva.
We developed polarization fluoroimmunoassays for 11-deoxycortisol in serum and saliva. To avoid interfering factors, the steroid was initially extracted from the biological fluids with dichloromethane. Assays could then be completed without any further separation procedures or need to correct for blank signals. The serum assay was suitable for following the response to the metyrapone test and r...
متن کامل11-deoxycortisol is a corticosteroid hormone in the lamprey.
Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide evidence that 11-deoxycortisol is the corticosteroid hormone in the lamprey, a m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1982
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.30.2127